Exploring Gene Expression Through Transformation Kit (with voucher)

Intermediate—Easy to perform; requires some background knowledge.
Product Highlights:
 
Explain the importance of promoters and gene regulation
Perform a successful bacterial transformation
Describe the role of transformation in genetic engineering
Articulate the importance of the controls in an experiment
 
Using β-thalassemia as a medical example, students explore the phenomenon of gene regulation in this hands-on kit. As students progress through the lab, they will answer why changes in the DNA sequence in front of a gene (not in the gene's coding sequence) affect how much functional protein is produced by the gene. 
 
They will also use a new plasmid in a transformation experiment that includes a gene for kanamycin resistance. They learn to utilize IPTG as a compound to turn on gene expression for certain promoters. Finally, students will successfully transform plasmid to produce green fluorescent protein (GFP). Kit includes enough materials for a class of 32 students working in 8 groups of 4, as well as 1-year access to digital resources that support 3-dimensional instruction for NGSS.
 
Time Requirement
Teacher preparation, 1-3/4 hours. Prelab, 45 minutes. Practice digital exercise, 10 minutes. Investigation: Introduction, 45 minutes; procedure, 50 minutes. Analysis questions, 45 minutes. Assessment questions, 45 minutes.
 
Performance Expectations
HS-LS1-1 From Molecules to Organisms: Structures and Processes. Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins which carry out the essential functions of life through systems of specialized cells.
 
Note: Kit includes a voucher to request perishable material later at your convenience. Contact us or return the voucher to request delivery of perishable materials.
 
 
Specifications - Shipping Information or Purchase Restrictions
This item contains living or perishable material and ships via 2nd Day or Overnight delivery.

What’s Included:
1 E. coli DH10B Slant Culture
2 Kanamycin Solutions, 4 mL, Vial
1 IPTG, 40 mg
1 GFP Plasmid, 200 µL
18 Sterile Graduated Transfer Pipets
3 Glass Beads, Pack
50 Sterile Petri Dishes, 100 x 15 mm
3 LB Agar Bottles, 400 mL
1 Carolina® UV LED Flashlight (356-nanometers wavelength)
1 Sterile Distilled Water, 1 mL
28 Disposable Inoculating Loops
30 Sterile Bulb Pipets, 1 mL
1 Nichrome Wire Inoculating Loop
18 Sterile Microcentrifuge Tubes, 2 mL
8 Calcium Chloride, 1 mL, Vials
1 Carolina Science Online® Resources (1-year subscription)
1 Teacher's Manual with Reproducible Student Guide