Purification of Green Fluorescent Protein 8-Station Kit, Module 2 (purification)

Intermediate - Requires some background knowledge.
 
This kit module is great for teaching:
The molecular basis of heredity
The relationship between genes and proteins
How E. coli is used to engineer proteins useful in medicine and manufacturing
Three techniques - transformation, protein purification, and protein gel electrophoresis - commonly used in biological research and its areas of application
 
This lab activity is part of a series set up in 3 modules that can be purchased separately and used in conjunction with one another. Successful completion of Module 2 is dependent on prior results from Module 1, Green Gene Colony Transformation Kit (item #211080, #211080P, #211082, or #211082P).
 
In this activity students take their successfully transformed bacteria and use HIC resin to conduct a batch purification technique. This purification process doesn't require the time or expense typically associated with column-based purification protocols and instead produces quick results in 1 class period (40 minutes).
 
Note: Kit includes a voucher to request perishable material later at your convenience. Contact us or return the voucher to request delivery of perishable materials.
 
Specifications - What’s Included:
1 Ampicillin, 4 mL (10mg/mL)
8 HIC Bead Resin, 300 µL
1 Equilibration Buffer, 10 mL
8 Lysis Buffer, 600 µL
8 Binding Buffer, 400 µL
8 Wash Buffer, 600 µL
8 TE Buffer, 600 µL
1 Luria Broth (LB), 20 mL
8 Culture Tubes
48 Microcentrifuge Tubes, 1.5 mL
1 Teacher’s Manual with Reproducible Student Guide
 
Needed But Not Included:
1 +pGREEN GFP Plate (from Module 1)
1 Shaking Water Bath or Incubator
 Pipets, 100-1,000 µL
 Pipet Tips
1 Microcentrifuge
 Permanent Markers
 Ice Buckets
 UV Light Source
 Waste Containers
 Vortex (optional)