PTC Extraction and Amplification Kit with 0.2-mL Tubes (with voucher)
SKU: 211377
S$606.00Price
Excluding Sales Tax
Product Highlights
- Exploring students inherited ability to taste the bitter chemical PTC
- Kit designed for 25 reactions
- Perishable set, HaeIII enzyme, and PTC tasting primers available separately
Product Details
Advanced—For experienced high school and college classes; requires some basic technical skill.
This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist, Albert Blakeslee.
Students will:
- Determine their ability to taste PTC using taste paper
- Use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA
- Amplify a 221-nucleotide region of the PTC taste receptor gene
- Perform restriction digestion to differentiate the 2 alleles by gel electrophoresis
- Determine how well the SNP genotypes actually correlate to tasting
Product Features
- PuReTaq™ Ready-to-Go™ PCR Beads
- Kit equips 25 students
Note: Kit includes a voucher to request perishable materials later at your convenience. Contact us or return the voucher to request delivery of perishable materials
SpecificationsWhat’s Included:
- 1 pBR322/BstNI Markers, 0.075 μg/μL, 200 μL
- 1 HaeIII Restriction Enzyme, 40 μL
- 1 PTC Primer/Loading Dye Mix, 700 μL
- 1 Chelex® Resin, 1.5 g
- 25 Ready-to-Go™ PCR Beads (0.2 mL tubes)
- 1 Mineral Oil, 5 mL
- 100 PTC Taste Strips
- 100 Control Taste Strips
- 1 Teacher's Manual and Reproducible Student Guide
Needed But Not Included:
- Saline Solution, 0.9%
- Micropipets and Tips, 1 μL to 1000 μL
- Micropipets and Tips, 1 μL to 1000 μL
- Microcentrifuge Tubes, 1.5 mL
- Microcentrifuge Tube Racks
- Microcentrifuge for 1.5 mL Tubes
- PCR Tubes, 0.2 or 0.5 mL
- Tube Adapters for Microcentrifuge, 0.2 or 0.5 mL
- Programmable Thermal Cycler
- Electrophoresis Chambers
- Electrophoresis Power Supplies
- Gel-staining Trays
- UV Transilluminator or White Light Box
- Paper Cups
- Permanent Markers
- Contrainer with Cracked or Crushed Ice
- Boiling Water Bath (optional)
- Camera (optional)
