Green Gene Colony Transformation Teacher Demonstration Kit, Module 1 (voucher)

An eye-catching example of the genetic basis of inheritance
 
Green color is clearly visible in normal classroom light
No UV lighting needed
Pre-aliquoted and prepared reagents minimize teacher prep time
 
Designed to match traditional AP® Biology Lab 6. Open your students' eyes to the genetic basis of inheritance with this simple lab. Developed in cooperation with the DNA Learning Center, this kit clearly demonstrates the induced uptake of a jellyfish gene by E. coli. Students observe the phenotypic effect of adding the gene for GFP (green fluorescent protein). GFP is expressed on plain LB—no color development substrate or induction reagent is needed. Your students can easily view the "green" effects in normal classroom lighting. Colonies will fluoresce under UV light. The plasmid containing the GFP gene also contains an ampicillin resistance gene, so ampicillin can be used to select for transformants. Lab procedure is easy to follow. The Teacher Demonstration Kit includes enough materials for 1 demonstration. Includes voucher to request perishable materials later at your convenience.
 
AP® is a trademark registered and/or owned by the College Board, which was not involved in the production of, and does not endorse, this product.
 
Specifications - What’s Included:
1 E. coli Culture
1 pGREEN Plasmid Solution, 200 µL
3 LB Agar Plates, Pre-poured
2 LB Agar Plates with Ampicillin, Pre-poured
1 50 mM Calcium Chloride, 3 mL
1 LB Broth, 3 mL
2 Transformation Tubes, 15 mL
7 Sterile Transfer Pipets, 1 mL
1 Glass Beads, 1 oz
4 Sterile Transfer Loops
1 Wire Inoculating Loop
1 Teacher's Manual and Reproducible Student Guide
 
Needed But Not Included:
1 Beaker, 600 mL
1 Container (for glass beads)
1 Culture Tube Rack
1 Wax Marker
1 Bunsen Burner
1 Water Bath, 42°C
1 Autoclavable Disposable Bag
 Ice
 Bleach Solution, 10%
 Incubator (optional)